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[Preprint]. 2023 Feb 24:2023.02.23.529824. [Version 1] doi: 10.1101/2023.02.23.529824

Fig. 7. Round spermatid formation is compromised in Tex10 cKO mice.

Fig. 7.

(A) Schematic representation of mouse spermatogenesis. An orange arrow indicates the stage for Stra8-iCre to play effect. Sg for spermatogonia, Sct for spermatocyte, and RS for round spermatid.

(B) Representative flow cytometry isolation of spermatogenic populations in mouse testes (left) and quantification data (right). N = 10 biological replicates per condition, and an unpaired t-test was used to detect significance.

(C) Expression changes of round spermatid markers in WT (Tex10 f/f) vs. cKO (Tex10 f/f;Stra8-iCre/+) mouse testes. The p-value was computed by edgeR using a generalized linear model-based statistical method.

(D) The scRNA-seq data are colored by WT (Tex10 +/+;Stra8-iCre/+, left panel, blue) and cKO (Tex10 f/−;Stra8-iCre/+, left panel, pink), and the scRNA-seq identifies cell populations from mouse testis (right panel), and n = 2 biological replicates per condition. t-SNE, t-distributed stochastic neighbor embedding.

(E) Cell proportion changes in WT (Tex10 +/+;Stra8-iCre/+) vs. cKO (Tex10 f/−;Stra8-iCre/+) mouse testes. Sg for spermatogonia, Sct for spermatocyte, RS for round spermatid, preSza for pre spermatozoa, Sza for spermatozoa, Peri for peritubular, and Sertoli for Sertoli cells.

(F) A summary model of Tex10 in controlling PGC specification and spermatogenesis. Under the wildtype condition, Tex10 binds the promoters and upregulates the expression of Psmd3/7, negative regulators for Wnt signaling, leading to the attenuation of the Wnt pathway (upper panel). Upon Tex10 depletion, the downregulation of Psmd3/7 expression levels hyperactivates Wnt signaling, resulting in compromised PGCLC specification in vitro and spermatogenesis in vivo (bottom panel).