Skip to main content
[Preprint]. 2023 Oct 24:2023.02.23.529711. Originally published 2023 Feb 23. [Version 2] doi: 10.1101/2023.02.23.529711

Figure 2. Relative expression of rbfox family mRNAs in adult brains and 3dpf larvae from the rbfox1sa15940 line.

Figure 2.

Adult brains: Relative brain expression of A) rbfox1, B) rbfox1l, rbfox2, rbfox3a and rbfox3b mRNA in adult brains. mRNA expression is normalised to the average expression of the mRNA in wild-type fish and to the reference housekeeping genes ribosomal protein L13a (rpl13) (A and B) or the eukaryotic translation elongation factor 1 alpha 1a (eef1a1a) (B). Kruskal-Wallis test followed by Dunn’s multiple comparison test. n = 5–7 WT, 5–7 HZ, 5–7 HOM. Mean ± SD. C) Brain expression of the rbfox family mRNAs in adult wild-type zebrafish brains represented as the quantification cycle value for each gene. n = 7 WT, 7 HZ, 7 HOM. Mean ± SD. 3dpf larvae: Relative brain expression of D) rbfox1, E) rbfox1l, rbfox2, rbfox3a and rbfox3b mRNA in 3 dpf whole larvae. mRNA expression is normalised to the average expression of the mRNA in wild-type fish and to the reference housekeeping gene ubiquitin-conjugating enzyme E2A (ube2a). Kruskal-Wallis test followed by Dunn’s multiple comparison test. n = 5 WT, 5 HZ, 5 HOM. Mean ± SD. F) Brain expression of the rbfox family mRNAs in 3 dpf wild-type larvae represented as the quantification cycle value for each gene. n = 5 WT, 5 HZ, 5 HOM. Mean ± SD. Each point represents the results from a pool of 10 larvae.