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[Preprint]. 2023 Feb 26:2023.02.26.530068. [Version 1] doi: 10.1101/2023.02.26.530068

Figure 3: SV-lysosomes in lrk-1 and apb-3 mutants are dependent on UNC-104.

Figure 3:

(A) Schematic showing the PLM neuron. Red boxes highlight the regions of imaging.

(B) SNG-1::GFP in the cell body, process, and synapses of PLM neurons showing dependence on UNC-104 in lrk-1(km17) and apb-3(ok429) mutants and their doubles with unc-104(e1265tb120). Scale bar: 10 μm.

(C) CTNS-1::mCherry in the cell body, process, and synapses of PLM neurons showing dependence on UNC-104 in lrk-1(km17) and apb-3(ok429) mutants and their doubles with unc-104(e1265tb120). Red arrows highlight fainter CTNS-1 puncta. Scale bar: 10 μm.

(D) Quantitation of co-transport of SNG-1 and CTNS-1 in unc-104(e1265tb120), lrk-1(km17); unc-104, and apb-3(ok429); unc-104 from kymograph analysis of sequential dual color imaging done at 1.3 fps. #P-value ≤ 0.05 (Mann–Whitney Test, all comparisons to WT); ns: not significant; Number of animals (N) ≥ 18 per genotype; Number of vesicles (n) > 1200.