Table 1.
Langmuir modelinga
| SOSIP.664 trimer | Fab | Affinity purification | konb (1/Ms) | koffb (1/s) | KDb (nM) | S m b |
|---|---|---|---|---|---|---|
| BG505 | PGT145 | 2G12 (n = 3) | 1.8. 104 ± 3.7. 102 | 5.1. 10−4 ± 2.0. 10−6 | 29 ± 0.69 | 0.74 ± 6.8. 10−3 |
| PGT151 | 2G12 (n = 2) | 8.7. 104 ± 3.5. 102 | 1.8. 10−4 ± 5.0. 10−7 | 2.0 ± 1.3. 10−2 | 1.9 ± 7.5. 10−3 | |
| B41 | PGT145 | 2G12 (n = 3) | 2.0. 104 ± 8.8. 102 | 1.2. 10−4 ± 1.5. 10−5 | 6.2 ± 0.28 | 0.63 ± 1.6. 10−2 |
| PGT145 (n = 3) | 3.2. 104 ± 2.3. 103 | < 10−5 | <0.5 | 0.87 ± 4.8. 10−2 | ||
| PGT151 | 2G12 (n = 3) | 4.0. 104 ± 4.1. 103 | 3.1. 10−3 ± 2.4. 10−4 | 77 ± 5.0 | 0.45 ± 1.5. 10−2 | |
| PGT145 (n = 2) | Minimal binding: < 10 RU | |||||
a
Tabulated values are means ± S.E.M of n independent replicates
b
kon and koff are the on- and off- rate constants, respectively; KD is equilibrium dissociation constant = koff/kon. Sm is stoichiometric number. Sm denotes the stoichiometry, i.e., number of Fab molecules per trimer.