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. 2023 Feb 20;2023:4967544. doi: 10.1155/2023/4967544

Figure 6.

Figure 6

YYD suppresses NSCLC cell proliferation. (a) A549 and H1975 cells were treated with different doses of YYD for 24 or 48 h. Then, cell viability was assessed by CCK-8 assay. (b, c) EdU and colony formation assays were used to determine the cell proliferation in the presence of specified doses of YYD for 48 h. (d) After treatment with indicated concentrations of YYD for 48 h, cells were subjected to cell cycle analysis. (e) Western blot assays of p53, p21, and cyclin D1 in YYD-treated cells. (f) Flow cytometry analysis of apoptosis in cells treated with YYD. (g) Apoptosis-related proteins in YYD-treated cells were quantified by western blot. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus the nontreated group.