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The p180 subunit of dCAF-1 interacts with the p55 and p105 subunits. Sf9 cells were coinfected with the indicated recombinant baculoviruses, and the proteins were purified by either anti-FLAG or Ni(II) affinity chromatography. The resulting protein preparations were subjected to SDS-polyacrylamide gel electrophoresis and staining with Coomassie brilliant blue R-250. The diagram at the bottom of the figure depicts the arrangement of the dCAF-1 subunits, as deduced from these experiments.
