TABLE 3.

ssODNs used for homology-directed repair

Elovl4 mutation	Sequence
Q180P	5′-CTAGGTAAACCTACTCCCACCCACATGGGGGTTCAGACAGAAAGGTGGGTCCCTCACCGGGCCCTCCAGCCACCCACTTGATTCCA ATCCACCACAGAGTGAACATGGTGCAGTGGTGGT-3′ (ApaI)
W246G	5′-GTAGAAGTTGAGGAAGAGGAAGATGAAGCTGATGGCGTAGGCGATCAGAGCCCAGTGCATGCCCTTGGGGAAGGGGCAGTC GGTGTAGAGAGACAGTGCTGTGTGTCCGATGGTCACGTGGAA-3′ (SphI)

The restriction enzymes used for genotyping KI cells are noted in parentheses, with their sequences underlined.