Figure 1.

Co-culture with M2-polarized macrophages enhanced the resistance of gastric carcinoma cells to OXA. Human monomyelocytic leukemia (THP-1) cells were stimulated to obtain M2 polarized macrophages. (A) the differentiation of macrophages was detected by flow cytometry. (B) the mRNA expression levels of CD206, Arg1, IL-10, and TGF-β in macrophages were detected by qRT-PCR. *P <0.05 vs. Un-Mac. (C) the cell viability was determined by CCK8. *P <0.05 vs. 24 h or 48 h. (D) the cell apoptosis was detected by flow cytometry. *P <0.05 vs. Un-Mac. B:Unpaired t test; C: Two-way ANOVA followed by Tukey's multiple comparisons test; D: Brown-Forsythe and Welch's ANOVA test followed by Unpaired t with Welch's correction.