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Solubility of ORO in different solvents. A: 15 ORO solutions show different degrees of red, representing different ORO dissolving abilities of 15 solvents. B. The absorbance at 492 nm of 15 ORO staining solutions semi-quantitatively reflects the solubility of ORO in different solvents. (n = 3. Error bars indicate mean ± SD. ns: p > 0.05, **P < 0.01; ****P < 0.0001, by ANOVA for multiple comparisons).
