FIG. 1.
DNA cyclization is enhanced in the presence of NE and rHMG-2. (A) DNA duplexes (21 bp) used in this study. Duplex 1 is predicted to have a linear geometry. Duplex 2 contains an A6 tract (boldface) that induces ∼18° of axial curvature. (B) DNA multimerization-cyclization assay. Gel electrophoretic analysis of circular ligation products obtained after incubation of either duplex 1 or duplex 2 under the indicated conditions. The 147-bp (seven 21-mer units) DNA circle is indicated (*). (C) Quantitation of DNA cyclization. Data are plotted as the normalized circle yield, Dnorm, versus the size of the DNA circle (21-mer units) in the absence (open) or presence (filled) of NE for duplex 1 (circles) and duplex 2 (triangles).