FIG. 2.

Schematic design of cyclization probes. Probes were based on transcription templates and contain two tandem 20-bp sequences carrying Gal4 binding sites (G) and the TATA box derived from the adenovirus E4 promoter (T). Probes have Acc65I restriction sites at each terminus. (A) Phasing probes contain a variable-length spacer (n). Curved probes contain four phased A₅ tracts (A). This curvature was replaced with a scrambled sequence of identical length in the uncurved templates. (B) Long phasing probes.