Figure 2.

Binding of the IgG1 to T18.1 in the pilus structure. (a). Flow cytometric binding of anti-T18.1 chimeric IgG1 clones and a negative non-binding antibody control (Control) to L. lactis PilM18 at 4°C or 37°C shown as a histogram. MFI for each condition is indicated above the peaks. Anti-human IgG Alexa Fluor 488 was used to detect binding and histograms were generated using FlowJo software. Data shown is representative of two independent experiments. (b) Bactericidal activity of vaccinated animal sera (rabbit anti-T18.1 and mouse anti-pilM18) and chimeric IgG1 clones against GAS (M217/T18.1) compared to control naïve rabbit serum. Data combines three independent experiments and is shown as mean ± SEM. For statistical analysis CFU data were log transformed and analysed by one-way ANOVA with Holm-šídák’s multiple comparisons test.