Table 3.
Information of PCR and sequencing primers.
| Primer | Sequence (5’-3’) | Application |
|---|---|---|
| UCHL1-Fw | TTCCTGTGGCACAATCGGAC | qRT-PCR primer for UCHL1 |
| UCHL1-Rv | CATCTACCCGACATTGGCCTT | |
| COL1A1-Fw | GGGATTCCCTGGACCTAAAG | qRT-PCR primer for COL1A1 |
| COL1A1-Rv | GGAACACCTCGCTCTCCA | |
| COL3A1-Fw | CTGGACCCCAGGGTCTTC | qRT-PCR primer for COL3A1 |
| COL3A1-Rv | CATCTGATCCAGGGTTTCCA | |
| Fibronectin-Fw | TCAACTCACAGCTTCTCCAA | qRT-PCR primer for Fibronectin |
| Fibronectin-Rv | TTGATCCCAAACCAAATCTT | |
| CNN1-Fw | CCAACCATACACAGGTGCAG | qRT-PCR primer for CNN1 |
| CNN1-Rv | TCACCTTGTTTCCTTTCGTCTT | |
| CDH1-Fw | CATTGCCACATACACTCTCTTCT | qRT-PCR primer for CDH1 |
| CDH1-Rv | CGGTTACCGTGATCAAAATCTC | |
| GAPDH-Fw | CCACATCGCTCAGACACCAT | qRT-PCR primer for GAPDH |
| GAPDH-Rv | GCGCCCAATACGACCAAAT | |
| dCas9-Fw | AATGGCATCCGAGACAAGCA | qRT-PCR primer for dCas9 |
| dCas9-Rv | TGTGCTCGTGAAGACTGTCC | |
| UCHL1-pyro-Fw | GGTTTTGTTTTTGTTTTTTTTGTATAGG | PCR and sequencing primer for UCHL1 pyrosequencing (lower cases reflect the universal primer, Y is the CpG sites tested, subscript number indicating the site) (Site #4 is SNP-rs577696101-C/G according to UCSC) |
| UCHL1-pyro-Rv | gggacaccgctgatcgtttaAATCTCCA-TCYACTTAAACTACATCTTC | |
| Pyroseq-sequencing primer | TTGTATAGGTTTTATAGTG | |
| Pyroseq-sequence to analyse |
Y1GTTTGGTY2GGY3GTTTTATAGTTGTAGTTTGGGY4GGTTTY5G TTAGTTGTTTTTY6GTTTTTTTTAGGTTATTTTTGTY7GGGYGTTTY GYGAAGATGTAGTTTAAGTYGATGGAGATT |