Figure 7.

Fructose increases intracellular Amp. (a) Intracellular Amp between WT-GBS and AR-GBS. (b) Intracellular Amp of AR-GBS being treated with indicated concentrations of fructose plus Amp. (c) Intracellular Amp of AR-GBS being treated with 20 mM or fructose plus 3-bromopyruvate or shikonin. (d) PMF of AR-GBS in the absence and presence of fructose. (e) Percent survival of AR-GBS in the presence of fructose plus Amp and effect of m-chlorophenyl hydrazone (CCCP). (f) Intracellular Amp of AR-GBS in the presence of fructose plus Amp or plus CCCP. Results are displayed as mean ± standard errors of the means (SEM) (N ≥ 3 technical replicates per sample), and statistically significant differences are identified by t-test. *, p < 0.05, **, p < 0.01. Each experiment was repeated independently at least three times.