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. 2022 Nov 3;18(1):2139986. doi: 10.1080/15592294.2022.2139986

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Figure 2. — Expression of histone modifying enzymes and activity is altered in SVs following neonatal DES exposure. Impact of DES treatment in WT and αERKO mice on genes associated with the following types of histones modifying genes (a-d). (a) Lysine methyltransferases (Kmts). (b) Lysine demethylases (Kdms). (c) Lysine acetyltransferases (Kats). (d) Lysine deacetylases (Hdacs). Heat maps of gene expression (log2 normalized FPKM) were plotted for each indicated category at week 5 and week 10. (e) Real time RT-PCR of Ezh2 in SV at 10 weeks in WT-veh, WT-DES, αERKO-veh and αERKO-DES (n = 4 per group). Numbers shown are Ezh2 expression normalized to 18S and then fold change calculated using the average of the WT-veh group set to 1. *p-value<0.01 using Mann Whitney. (f) ELISA assay analysis on H3K9ac. (g) Histone H3K27me3, H3K27ac and total H3 immunoblots for each indicated category at week 10 (n = 3 individual mice per group). H3K27me3 and H3K27ac were normalized to total H3, and values plotted in graphs.