Figure 4.

Mid1p-dependent actin-myosin contractile ring (ACR) assembly in S. pombe. (a) Phosphorylation of Mid1p by Plo1p kinase triggers its release from the nucleus. (b) Type-I interphase nodes form by sequential recruitment of Mid1p, Cdr1p and Cdr2p, they interact with the cell membrane through the PH and C2 domains of Mid1p. (c) Type-II interphase nodes form by sequential recruitment of Gef2p, Blt1p, Klp8p and Nod1p, they interact through the membrane binding domain of Blt1p. (d) Cytokinesis nodes form when Type-II interphase nodes are captured by Type-I nodes after they migrate to the medial cortex, and upon the onset of mitosis, Myo2p, Rng2p, Cdc12p and Cdc15p are recruited to interphase nodes, cytokinesis nodes then condense into the actin-myosin contractile ring. See main text and Table S1 for references and mammalian homologue proteins. .