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. 2023 Mar 2;18(3):e0282223. doi: 10.1371/journal.pone.0282223

Fig 6. Acss2/HIF-2 regulates cancer-associated factors in HT29 cells.

Fig 6

Semi-quantitative RTPCR measurements of HIF-1 selective (PGK1) target genes in comparison with HIF-2 preferential or HIF-1/HIF-2 co-regulated (VEGFa, PAI1, MMP9, GLUT1) target genes in (A) HIF-2α knockdown HT29 cells rescued with wild-type (K3) or arginine-lysine substituted mutant (R3) HIF-2α, or (B) Acss2 knockdown HT29 cells rescued with wild-type (WT) or cytosol-restricted mutant (ED) Acss2, after the indicated period of hypoxia exposure or glucose deprivation. Comparison of samples within a given condition was performed by one-way ANOVA followed by Dunnett’s multiple comparisons test using control shRNA knockdown/control rescue as reference with decreased samples noted (*, P<0.05). Values indicated are means with SD.