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. 2023 Feb 16;17:1117845. doi: 10.3389/fnins.2023.1117845

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Copyright © 2023 McQuaid, Solorzano, Dickerson and Deane.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Concentration-dependent SARS-CoV-2/SP uptake by cerebrovascular cells. (A) SP-555 uptake at different SP-555 concentration for the hCMEC/D3 (green), HBVP (red), and HBVSMC (purple) using curve fitting. (B) Binding constant (Kd) for HBVSMC that was best fitted to a saturable uptake. There was no saturation for the other cell types over the concentrations used. Values are mean ± SEM. N = 3–5 wells/group Au = Fluorescence arbitrary unit. Supplementary Figures 1, 2.