Extended Data Fig. 7. Targeted proteomic analysis of producer/consumer status of live/dead sorted populations after amphotericin B treatment.
a, Plots illustrating gating strategy for FACS of cells originating from heterogeneous ¹³C-labelled colonies challenged with amphotericin B and stained with propidium iodide (PI). 10,000 events were recorded to check viability and gating of each sample, after which a minimum of 4 million cells were sorted per population. Singlets were gated based on FSC-A (forward scattered channel - area) and FSC-H (forward scattered channel - height) intensities. Conservative live and dead population gates were defined based on the FSC-A and 610 nm channels. b, Quantification of producer and consumer peptides in live and dead populations obtained by FAC-sorting. Each plot shows the data for a single peptide, quantified at the precursor level using a targeted proteomics (PRM) workflow. Dead cell populations consistently contain fewer peptides originating from producer cells.