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. 2023 Feb 16;8(3):441–454. doi: 10.1038/s41564-022-01304-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — a, Plots illustrating gating strategy for FACS of cells originating from heterogeneous ¹³C-labelled colonies challenged with amphotericin B and stained with propidium iodide (PI). 10,000 events were recorded to check viability and gating of each sample, after which a minimum of 4 million cells were sorted per population. Singlets were gated based on FSC-A (forward scattered channel - area) and FSC-H (forward scattered channel - height) intensities. Conservative live and dead population gates were defined based on the FSC-A and 610 nm channels. b, Quantification of producer and consumer peptides in live and dead populations obtained by FAC-sorting. Each plot shows the data for a single peptide, quantified at the precursor level using a targeted proteomics (PRM) workflow. Dead cell populations consistently contain fewer peptides originating from producer cells.