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. 2023 Feb 16;8(3):441–454. doi: 10.1038/s41564-022-01304-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — Thirty thousand events were recorded per sample (fewer for the controls). Singlets were gated based on FSC-A (forward scattered channel - area) and FSC-H (forward scattered channel - height) intensities. The median singlet rate across the 24 samples was 92.8% with a minimum of 90.5%. Live and dead population gates were defined based on the live and dead controls using FSC-A and 670 nm channels. Identical gates were applied to all samples. Three samples are shown as examples.