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. 2023 Mar 2;13:3529. doi: 10.1038/s41598-023-30553-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis and biochemical detection of anti-metastatic tumor potential. (a) Microscopic images of Ki-67, CD44, and caspase 3-immunostained mammary tumor as well as Ki-67-immunostained liver sections of the untreated mammary tumor-bearing mouse group (MT), CD NPs-treated MT group (MT-CD NPs), MT-ZD NPs group, and MT-Fu group with (b,c) their percentages of positive immunostained tumor cells. (d) The blood level (ng/mL) of α-fetoprotein (AFP). All values are demonstrated as mean ± SEM. MT-CD NPs group was compared to other groups, considering significantly different at p < 0.05*, < 0.01**, and < 0.001***.