Skip to main content
. 2023 Feb 1;55(2):333–346. doi: 10.1038/s12276-023-00929-x

Fig. 3. A p62 ligand exerts inhibitory effects against various innate stimuli.

Fig. 3

a–d PMs were pretreated with ATB1021 (10 μM) for 1 h and stimulated with zymosan (10 μg/mL; a, b) or poly(I:C) (10 μg/mL; c, d) for the indicated times; they were then analyzed by quantitative RT‒PCR to determine the mRNA levels of Il1b and Il6 (a, c; n = 3) or by ELISA to determine the protein level of IL-6 (b, d; n = 4). e mRNA levels of Cxcl5, Il18, Cxcl9, Cxcl10, Il1b and Il6 in lung tissues from mice instilled with poly(I:C) (10 mg/kg, intranasally) and treated with vehicle or ATB1021 (20 mg/kg; intraperitoneal) (n = 6). f, g H&E staining (f) and inflamed areas (g, n = 3) in the lungs of the mice treated as in e. Means ± SDs (a–d) and ±SEM are shown (e, g). Statistical analysis was performed by two-tailed Student’s t test (a–d) or the Mann‒Whitney U test (e, g). n.s., not significant, *p < 0.05, **p < 0.01, ***p < 0.001.