Figure 6.

Inulin treatment is as efficient as anti-PD-1 immunotherapy. (A) Experimental schedule. C57BL/6 mice were fed with a control diet or inulin-enriched diet (7.2% in drinking water) (n=12 mice per group) starting 15 days before s.c. inoculation of 2x10⁵ B16 OVA melanoma cells. Anti-PD-1 immunotherapy was injected i.p. to 6 mice per group on days 4, 7 and 10 post-tumor inoculation. (B) Mean and (C) individual tumor growth curves of mice treated as described in (A). (D–F) Frequency of B16 OVA tumor-infiltrated IFNγ-producing cells gated on CD45+ CD3+ γδ TcR- CD4+, defined as CD4⁺ T cells (C), CD45+ CD3+ γδ TcR- CD4-, defined as CD8⁺ T cells (D) or CD45+ CD3+ γδ TcR+, defined as γδ T cells (E) from mice treated as described in (A). Graphs show the mean ± SEM. ns = not-significant, *p < 0.05, **p < 0.005, ***p < 0.001 by two-way ANOVA with Geisser greenhouse’s correction (B) or by Mann-Whitney tests (D–F).