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. 2023 Feb 17;15:1098034. doi: 10.3389/fnagi.2023.1098034

Figure 2.

Figure 2

Loss of angiotensin-converting activity in recombinant F-and C-ACE from PS1-KO fibroblasts. Ang-converting (angiotensin-converting) activity was measured by incubating 2 μg of ACE protein with the synthetic o-aminobenzoyl peptide substrate for 24 h at 37°C. (A) F-ACE purified from WT fibroblasts showed Ang-converting activity, whereas PS1-KO fibroblasts did not show any Ang-converting activity. (B) N-ACE purified from WT and PS1-KO fibroblasts did not show any Ang-converting activity. (C) C-ACE purified from WT fibroblasts showed Ang-converting activity, whereas PS1-KO fibroblasts did not show any Ang-converting activity. Values represent the means ± SD; n = 3; ****p < 0.0001. NS, not significant, by unpaired two-tailed Student’s t test.