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. 2023 Feb 17;15:1098034. doi: 10.3389/fnagi.2023.1098034

Figure 4.

Figure 4

Transfection of PS1WT and PS1 mutants restored angiotensin-converting activity of F-ACE and C-ACE in PS1-KO fibroblasts. Ang-converting activity was measured by incubating 2 μg of ACE protein with the synthetic o-aminobenzoyl peptide substrate for 24 h at 37°C. (A) Transfection of PS1WT and PS1 mutants fully restored Ang-converting activity of F-ACE in PS1-KO fibroblasts. (B) N-ACE purified from WT, PS1-KO, and PS1-KO fibroblasts transfected with PS1WT and PS1 mutants did not show any ang-converting activity. (C) Transfection of PS1WT and PS1 mutants fully restored the Ang-converting activity of C-ACE in PS1-KO fibroblasts. PS1WT and PS1 mutants did not exert different effects on angiotensin-converting activity. Values represent the mean ± SD; n = 3; NS, not significant, ****p < 0.0001, Holm-Sidak’s multiple comparisons test.