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. 2023 Mar 2;13:3505. doi: 10.1038/s41598-023-30562-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SEC-MALS analysis of GRB2 mutants. Overlays of GRB2 WT, N188D/N214D, and V123D monomers are shown in black, orange, and blue, respectively, with (A) light scattering or (C) refractive index plotted against elution time. Overlays of GRB2 WT, N188D/N214D, and V122P/V123P dimers are shown in black, orange, and blue, respectively, with (B) light scattering or (D) refractive index plotted against elution time. Dashed lines denote the expected molecular weights of GRB2 monomer (~ 28 kDa) and dimer (~ 56 kDa).