Table 1.
Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of acidocin J1132β derivatives against 9 strains of pathogenic bacteria.
| Pathogenic bacteria | Culture medium | MIC (MBC) (µg/ml) | |||||
|---|---|---|---|---|---|---|---|
| A0 | A4 | A6 | A9 | A11 | Melittin | ||
| Gram-negative bacteria | |||||||
| Salmonella Typhimurium ATCC 13311 | TSB | > 250 (> 250) | 31.25 (31.25) | 62.5 (62.5) | 62.5 (62.5) | 15.63 (31.25) | 3.91 (3.91) |
| Pseudomonas aeruginosa ATCC 27853 | TSB | > 250 (> 250) | 250 (250) | 125 (250) | 250 (250) | 31.25 (62.5) | 15.63 (15.63) |
|
Shigella sonnei ATCC11060 |
TSB | > 250 (> 250) | 31.25 (31.25) | 62.5 (125) | 62.5 (125) | 31.25 (62.5) | 3.91 (7.81) |
|
Acinetobactero baumanii MT strain |
TSB | > 250 (> 250) | 15.63 (31.25) | 31.25 (31.25) | 62.5 (125) | 15.63 (31.25) | 7.81 (7.81) |
| GMa (Gr.- strains) | – | > 250 | 44.2 | 62.5 | 88.39 | 22.1 | 6.8 |
| Gram-positive bacteria | |||||||
| Staphylococcus aureus ATCC 25923 | TSB | > 250 (> 250) | > 250 (> 250) | > 250 (> 250) | > 250 (> 250) | > 250 (> 250) | 3.91 (3.91) |
|
Staphylococcus epidermidis ATCC 12228 |
TSB | > 250 (> 250) | 250 (250) | > 250 (> 250) | 62.5 (> 250) | 15.63 (31.25) | 3.91 (3.91) |
|
Bacillus cereus ATCC 11778 |
TSB | > 250 (> 250) | > 250 (> 250) | > 250 (> 250) | > 250 (> 250) | 250 (250) | 3.91 (7.81) |
| Listeria monocytogenes 10403s | TSBYE | > 250 (> 250) | 250 (> 250) | 250 (> 250) | 250 (> 250) | 125 (125) | 3.91 (3.91) |
| GMb (Gr. + strains) | – | > 250 | > 250 | > 250 | 125 | 78.75 | 3.91 |
| MHCc (µg/ml) | – | – | > 250 | > 250 | > 250 | > 250 | ˂ 0.98 |
| TId (Gr.- strains) | – | – | 11.31 | 8 | 5.66 | 22.62 | 0.07 |
| TIe (Gr. + strains) | – | – | 1.00 | 1.00 | 4.00 | 6.35 | 0.13 |
TSB tryptic soy broth, TSBYE tryptic soy broth supplemented with 0.6% yeast extract.
aGM (Gr.− strains) denotes the geometric mean of MIC values from all Gram-negative strains.
bGM (Gr.+ strains) denotes the geometric mean of MIC values from all Gram-positive strains.
cMHC is the minimum concentration that caused 10% hemolysis of human red blood cells (hRBC). When no 10% hemolysis, as well as > 10% hemolysis, were observed at 250 µg/ml and 0.98 µg/ml, values of 500 µg/ml and 0.49 µg/ml were used to calculate the therapeutic index, respectively.
dTherapeutic index (Gr.− strains) is the ratio of the MHC to the geometric mean of MICs from Gram-negative strains.
eTherapeutic index (Gr.+ strains) is the ratio of the MHC to the geometric mean of MICs from all Gram-positive strains.