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. 2023 Mar 2;14:1200. doi: 10.1038/s41467-023-36901-6

Fig. 5. TaWD40-4B.1C but not TaWD40-4B.1T enhances tolerance to oxidative stress and reduces H2O2 level under drought stress.

Fig. 5

a The assessment of the oxidative stress tolerance of SR3 and its transgenic lines. Bar = 2 cm. b The statistical result of plant height in a (n = 9 biologically independent samples; P = 1.30 × 10−106). c The H2O2 levels revealed by the DAB staining in the leaves of wheat seedlings under the well-watered and water-withheld conditions. Bar = 1 cm. d The MDA contents in the leaves of wheat seedlings under the well-watered and water-withheld conditions (n = 9 biologically independent samples; P = 1.58 × 10−84). In b–d, the box indicates the range from lower to upper quartiles, and the bar ranges the minimum to maximum observations; the significance of the difference is calculated with a one-way ANOVA analysis–Tukey comparison and the columns labeled without the same alphabet are significantly different (P < 0.05, two-sided). SR3: the cultivar carrying TaWD40-4B.1C; OE-T: TaWD40-4B.1T overexpression lines; OE-C: TaWD40-4B.1C overexpression lines; RNAi: TaWD40-4B.1C RNAi lines. Source data are provided as a Source data file.