Fig. 7. TaWD40-4B.1C enhances grain yields under the water-withheld condition.
a The representative seedlings under water-withheld condition. Bar = 10 cm. b The grain sizes of SR3 and its transgenic lines under the well-watered and water-withheld conditions. Bar = 1 cm. c, d The statistical comparison of grain width (c) and length (d) in b (n = 10 biologically independent samples; P = 1.48 × 10−51 in c and P = 5.13 × 10−42 in d). e The 1000-grain weights under the well-watered and water-withheld conditions (n = 10 biologically independent samples; P = 6.27 × 10−62). f–h The grain number per plant (f), grain number per spike (g), and plant height (h) under the well-watered and water-withheld conditions (n = 10 biologically independent samples; P = 1.06 × 10−17 in f, and P = 3.91 × 10−46 in g and P = 1.45 × 10−22 in h). i The grain yield of plants under the well-watered and water-withheld conditions (n = 10 biologically independent samples; P = 8.41 × 10−49). j–l The CO2 assimilation (j), transpiration rate (k), and water use efficiency (l) under the well-watered and water-withheld conditions (n = 10 biologically independent samples; P = 5.15 × 10−38 in j, and P = 6.45 × 10−11 in k, and P = 2.17 × 10−19 in l). In c–l, data are shown as mean and standard deviation, the significance of the difference is calculated with a one-way ANOVA analysis–Tukey comparison, and the columns labeled without the same alphabet are significantly different (P < 0.05, two-sided). SR3: the cultivar carrying TaWD40-4B.1C; OE-T: TaWD40-4B.1T overexpression lines; OE-C: TaWD40-4B.1C overexpression lines; RNAi: TaWD40-4B.1C RNAi lines. Source data are provided as a Source data file.