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. 2002 Jan 15;30(2):581–591. doi: 10.1093/nar/30.2.581

Figure 1.

Figure 1

The rhp23::ura4 mutant is sensitive to UV light but not ionizing radiation or HU. Cell survival after UV irradiation (A), ionizing radiation exposure (B) or HU treatment (C) of wild-type SP223, rhp23::ura4, rhp23::ura4 (pYZ1N-rph23) and rad9-192 cells. The rad9-192 mutant was used as a control since it is highly sensitive to all three agents (36). The rhp23::ura4 disruption does not cause increased sensitivity to ionizing radiation or HU, but mutant cells are moderately sensitive to UV light in comparison with the resistant wild-type SP223 strain and the highly sensitive rad9-192 mutant strain. The pYZ1N-rhp23 plasmid containing the rhp23 cDNA restores UV resistance to wild-type levels in the rhp23::ura4 disruption strain at both high (no thiamine) and low expression (plus thiamine; data not shown) levels. Experiments in (A) and (B) were performed in medium without thiamine (high expression) and the experiments in (C) were performed in the presence of thiamine (low expression). Error bars represent the standard deviation of three measurements.