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. 2002 Jan 15;30(2):581–591. doi: 10.1093/nar/30.2.581

Figure 6.

Figure 6

Figure 6

Rhp23-binding proteins. (A) Interacting proteins identified in the yeast two-hybrid system. The β-galacatosidase activity of strains carrying the indicated cDNAs in the yeast two-hybrid system was determined in triplicate by a plate assay and a liquid assay (42). The units column shows the mean and standard deviation for three measurements in the liquid assay. The positive control on the top line is HIV-1 Vpr and human UNG, which interact in the two-hybrid system (49). The negative control on the bottom line is rhp23 with the pGAD GH vector (ClonTech, CA) not containing an insert. (B) Alignment of Rhp23-binding proteins. The amino acid sequences were aligned using a multiple sequence alignment Jellyfish program (Biowire). Identical amino acids are in dark color. The highly similar and moderately similar residues are indicated by light color. Consensus amino acids are listed under each residue. Note that the first 76 amino acids, which correspond to the ubiquitin sequence, are the only homologous sequences shared by all four proteins.