Table 1. Schizosaccharomyces pombe strains and plasmids.
Name | Genotype | Source |
---|---|---|
Strains | ||
SP223 | Wild-type, h– ade6 leu1-32 ura4-294 | David Beach |
Rad9-192 | h– rad9-192 ade6 leu1-32 ura4-294, isogenic to SP223 | (36) |
Rhp23::ura4 | h– rhp23::ura4+ ade6 leu1-32 ura4-294, isogenic to SP223 | This study |
RE70 | A single copy integrant of a gfp–rhp23 fusion | This study |
RE74 | A single copy integrant of gfp | This study |
Plasmids | ||
pYZ1N | Derivative of pREP1N | (25) |
pYZ3N | pYZ1N with GFP | (25) |
pYZ3N-rhp23 | S.pombe gfp–rhp23 fusion | This study |
pYZ1N-rhp23 | S.pombe rhp23 in pYZ1N | This study |
pYZ1N-RAD23 | S.cerevisiae RAD23 in pYZ1N | This study |
pYZ1N-HHR23A | Human HHR23A in pYZ1N | This study |
pSF173-rhp23 | HA–rhp23 fusion | This study |