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Sprouty2ΔIEmice have increased numbers of Reg4+ DCS cells. (A) Distal colonic sections from Spry2ΔIE (ΔIE, n = 5) and control littermates (FF, n = 9) were analyzed by RNAScope in situ hybridization for the DCS cell marker Reg4, quantified in panel B. (C) Bulk RNA-seq on distal colonic tissue was analyzed for expression levels of DCS cell markers. n = 6 per genotype. Data were analyzed by 2-tailed Student t test. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. TPM, transcripts per million.
