Figure 3.
Retnlb/RELMβ is expressed in DCS cells at homeostasis and is up-regulated by deletion of Sprouty2. HDP expression was assessed in our bulk RNA-seq data set generated from distal colons of Spry2ΔIE and control littermates (GSE160854). (A) Expression of multiple HDPs (Defb1, Defb42, Defb45, Restn, Retnla, Retnlb, and Camp) was detected in the colon. (B) Analysis of HDP expression in cellular populations identified in colonic scRNA-seq showing enrichment of Defb1, Defb45, and Retnlb in the epithelium. (C) Colonic samples from a larger cohort of Spry2ΔIE (n = 9) and control (n = 12) littermates were analyzed by quantitative polymerase chain reaction (qPCR) to test for RELMβ (Retnlb) expression levels. Data were analyzed by 2-tailed Student t test. ∗P < .05. TPM, transcripts per million; UMI, unique molecular identifier.