Figure 4.

Sprouty2^ΔIEmice have activated ILC2s in the colon. (A) Transcriptomic analysis of ILC2s obtained from single-cell sequencing of colonic mucosal isolate from Spry2^ΔIE (n = 303 cells) and control (n = 136 cells) mice representing 1.12% and 0.51% of the total cells recovered for each genotype, respectively, and shown as uniform manifold approximation and projection (UMAP). (B) Violin plots showing expression of ILC2 identity genes (Gata3, Fgl2, and Ly6a) and ILC2 activation markers (Ramp1, Ramp3, Ifngr1, Anxa1, Socs1, and Csf2) in Spry2^ΔIE and control mice. (C) Analysis of Il13 expression in the ILC2 populations of Spry2^ΔIE and control mice by violin plot and UMAP. (D) Expression of IL13 receptors in bulk RNA-seq on distal colons on mice. (E) Cell-specific expression levels of the IL13 receptors, Il13ra1 and Il4ra, and inhibitory receptor, Il13ra2, were analyzed in the colonic scRNA-seq data set, showing broad expression of Il13ra1 and Il4ra across epithelial cell types, including DCS cells, and no expression of Il13ra2 in the epithelium. Data were analyzed by 2-tailed Student t test. TPM, transcripts per million; UMI, unique molecular identifier.