Figure 5.

ILC2s are the predominant source of IL13 in the colonic mucosa. (A) Uniform manifold approximation and projection (UMAP) plot of colonic epithelial and stromal cells subjected to scRNA-seq. (B) UMAP and population expression analysis showing Il13 expression largely restricted to the ILC and T-lymphocyte cluster. (C) Subclustering of the ILC and T-lymphocyte population shown by UMAP. (D) UMAP and population expression analysis showing Il13 expression is expressed predominantly in the ILC2 population, with minimal expression in other cell types. (E) Expression intensity dot map representing gene expression based on the fraction of cells that were positive and abundance of gene expression within each cluster in the ILC and T-lymphocyte subcluster. (F) In situ hybridization for Il13+ cells in the colonic epithelium. IEL, intraepithelial lymphocyte; NK, natural killer; UMI, unique molecular identifier.