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. 2022 Dec 26;3(2):100177. doi: 10.1016/j.xjidi.2022.100177

Figure 2.

Figure 2

Effect of inflammation on oxylipin concentrations in skin. Rats were injected (n = 24 per group) with CFA, a common, experimental inflammatory stimulus or PBS, intradermally. Behavioral responses as indicated were measured: (a) ipsilateral wiping, (b) bilateral wiping, and (c) scratching of the injection site (n = 24, 18, 10 per group 4 hours, 1 day, and 4 days after injection, respectively). The abundance of mediators as indicated in CFA-induced inflammation was compared with PBS injection, (d) 9-HODE (∗∗P < 0.0001 for 4 hours after CFA and 1 day after CFA), (e) PGE2 (∗∗P = 0.0006 for 1 day after CFA and ∗∗P < 0.0001 for 4 days after CFA), (f) 9,13-EHL (∗∗∗P < 0.0001 for 4 hours after CFA and 1 day after CFA), and (g) 9,10,13-THL (∗P = 0.048 for 4 hours after CFA). (h) The proportion of 9-HODE (∗P = 0.0220 for 1 day after CFA), 9,13-EHL, and 9,10,13-THL (∗P = 0.0184 for 4 days after CFA) present in the skin as free acid was measured 4 days and 1 day after CFA injection compared with that after PBS. Data are presented as mean ± SD. In panels a–c, no significant differences were found as determined by one-way ANOVA with a Holm-Šídák correction for multiple comparisons. Significance in panels d–h was determined using a two-way ANOVA with a Holm-Šídák correction for multiple comparisons. Each data point refers to one biological replicate; n ≥ 6. 9,10,13-THL, 9,10,13-trihydroxy-octadecenoate; 9,13-EHL, 13-hydroxy-9,10-epoxy octadecenoate; CFA, complete Freund’s adjuvant; HODE, hydroxyoctadecenoate; PGE2, prostaglandin E2.