Nocifensive response in rats after injection of vehicle, 9,10,13-THL, and PGE2. The number of (a) ipsilateral wipes and (b) ipsilateral and contralateral wipes, (c) the combined total number of wipes from panels a and b, and (d) the number of scratching bouts after injection of vehicle, 9,10,13-THL, or PGE2. No significant differences were found using a one-way ANOVA with a Holm-Šídák correction for multiple comparisons. Data are presented as mean ± SD, and each data point corresponds to one biological replicate; n = 8. 9,10,13-THL, 9,10,13-trihydroxy-octadecenoate; min, minute; PGE2, prostaglandin E2.