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. 2022 Dec 26;3(2):100177. doi: 10.1016/j.xjidi.2022.100177

Figure 6.

Figure 6

Peripheral sensitization induced by 9,10,13-THL in normal and knockout mice. (a) Changes in withdrawal latency on hot plate after 5-minute incubation of intraplantar injection of 9,10,13-THL (327 μM in 10 μl). For WT vehicle versus WT 9,10,13-THL, ∗∗P = 0.0047; for WT 9,10,13-THL versus everything else, ∗∗∗∗P < 0.0001. (b) Changes in withdrawal latency on cold plate after 5-minute incubation of intraplantar injection of 9,10,13-THL (327 μM in 10 μl). For WT vehicle versus WT 9,10,13-THL, ∗P = 0.0498; for WT 9,10,13-THL versus everything else, ∗∗∗∗P < 0.0001. (c) Changes in withdrawal force in the mechanical assay after 5-minute incubation of intraplantar injection of 9,10,13-THL (327 μM in 10 μl). No significant differences were found within groups when comparing treatment (vehicle vs. 9,10,13-THL) or between strains. (d) Changes in withdrawal latency in the dry ice assay after 5 minutes of incubation of intraplantar injection of 9,10,13-THL (327 μM in 10 μl). No significant differences were found within groups when comparing treatment (vehicle vs. 9,10,13-THL) or between strains. Data are presented as mean ± SD, and each data point corresponds with a single biological replicate; n ≥ 6. Significance was determined by comparing vehicle-injected with 9,10,13-THL‒injected behavior responses within each genotype. Significance was determined using a Mann‒Whitney U test when comparing within strains. A two-way ANOVA with the Holm-Šídák correction for multiple comparisons was used when testing for significance between strains and treatments. 9,10,13-THL, 9,10,13-trihydroxy-octadecenoate; ns, not significant; WT, wild type.