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. 2002 Jan 15;30(2):439–446. doi: 10.1093/nar/30.2.439

Figure 6.

Figure 6

Nuclease S1 protection analysis confirms the new mRNA 3′ end detected by the CR–RT–PCR. Protected products (lane 5) were coelectrophoresed with products obtained in control experiments and with DNA marker fragments (lanes M), whose sizes are given in the left margin. The control reactions are the antisense probe without any further treatments (lane 1), incubated under S1 conditions without nuclease and RNA (lane 2), incubated with 75 U S1 and without RNA (lane 3) or 40 µg of wheat germ tRNA products (lane 4). The size of the protected fragment of ∼100 nt corresponds to a 3′ end ∼300 bp downstream of the translation stop codon, which is consistent with the 3′ terminus mapped by the CR–RT–PCR analysis.