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. 2002 Jan 15;30(2):497–506. doi: 10.1093/nar/30.2.497

Figure 1.

Figure 1

Analyses of the interactions between dps from +1 to +90 and proteins in different types of rice nuclear extracts. EMSAs were performed using a labeled DNA probe from +1 to +90. (A) DNA fragments (dps, P1 or P2) and double-stranded oligonucleotides having overlapping sequences between +1 and +90 (dps1 and dps-a to dps-c) were used as cold competitors at a 200-fold molar excess in EMSAs. (B) EMSAs were performed without (lane 1) or with nuclear extracts from cell suspensions or shoots in the absence (–) or presence of competitors (dps, dps1, P1 or P2). DNA–protein complexes (C1 and C2) are indicated. P is free DNA probe. (C) EMSAs were carried out without (lanes 1 and 6) or with cell suspension or root nuclear extracts in the absence (–) or presence of competitors (dps, dps1 or dps-a to dps-c). DNA–protein complexes are designated C1–C3.