Figure 5.

Analysis of the DNA-binding proteins by DNA UV crosslinking. A radiolabeled DNA probe substituted with BrdU was incubated without (lane 1) or with nuclear extracts from rice shoots (S) or cell suspensions (C) in the absence (–) or presence of a 100-fold molar excess of the competitors indicated. UV crosslinked proteins were separated in a 14% SDS–polyacrylamide gel. The sizes of the marker proteins are indicated on the right. The arrowheads indicate the apparent molecular masses of the crosslinked proteins. Asterisks indicate incomplete digestion of the labeled DNA.