Figure 8.

GAGAG motifs contribute to RTBV promoter activity. (A) Nucleotide sequence and potential secondary structure of the dps3 transcript. (B) Schematic representation of the constructs tested in transfected O.sativa protoplasts showing the expanded dps3 region with deletions. A filled box indicates the TATA box. A bent arrow represents the transcription start site. Open boxes show the GAGAG motifs. Dashes in the sequences indicate identity with the wild-type sequence. Asterisks indicate deleted bases. Constructs and relative CAT activities are shown on the left. The relative CAT activity given for each construct is an average of at least three independent transient expression assays.