Figure 2.

Selection of RNA aptamers to recombinant gBP21. (A) (Insert) Schematic representation of the starting RNA pool (pool no. 1), which consists of a central 40 nt randomized sequence stretch flanked by primer binding sites that are predicted to fold into irregular hairpin structures. Either of the two hairpins can function as a binding site for gBP21 (14,35). Bar graph of the amount of bound RNA during 12 SELEX rounds. Maximal binding was achieved in round no. 10, which was cloned and further analyzed. (B) DNA sequences of six representative aptamers. C nucleotides are highlighted in orange and G nucleotides in green to emphasize the nucleotide bias of the selected aptamers. Primer binding sites are shown as gray boxes.