Figure 3.

GRN137159 binds to telomerase by association with hTR. Affinity purified telomerase was incubated with either ³²P-labeled GRN137159 (4000 c.p.m./fmol) or GRN137166 (2000 c.p.m./fmol), as indicated above, followed by analysis on a native gel (described in Materials and Methods). The position of the active telomerase RNP complex (lane 3) was used as a reference and obtained by incubating the enzyme with (GTTAGG)₃ and [α-³²P]dGTP. Duplicate samples were treated with proteinase K, where indicated. The labeled oligonucleotides were also allowed to bind in vitro synthesized hTR (lanes 7 and 8), which serves as a marker for free telomerase RNA.