Fig. 1.

Selenite-induced cell cycle arrest and cell death. A Cell viability and IC₅₀ of H1975 and H358 treated with selenite at 24, 48, and 72 h were assessed by MTS assay. B Flow cytometry revealed cell cycle arrest induced by sodium selenite at 72 h. C Western blot analysis of cleaved PARP and cleaved caspase 3 in 72-h selenite-treated cells demonstrated apoptosis. D Western blot analysis showed upregulation of cytochrome C, BIM, and NOXA, representative markers of the intrinsic pathway of apoptosis caused by 72-h selenite treatment