Table 2. Dissociation constants for binding of hisPTB-34s mutants to EMCV IRES domain 1 RNA.

Mutation	Kd (µM)	Structural location
Wild-type	0.083 ± 0.009	–
H411A/Q412A	0.14 ± 0.01	β4– β5 loop
R418A	0.20 ± 0.01	β4– β5 loop
Q424A	0.09 ± 0.01	β4– β5 loop

Data were fitted to a single-site binding model. Values quoted are the average of three experiments (± standard deviation).