Table 1. Primers used for qRT-PCR.
circRNA ID | Primers | Tm (° C) | Product size (bp) |
---|---|---|---|
GAPDH | F: 5′ CACTGAGCAAGAGAGGCCCTAT3′ R: 5′ GCAGCGAACTTTATTGATGGTATT3′ |
60 | 144 |
mmu_circRNA_35869 | F: 5′ ACACTGACATCGAGGGCATAGA3′ R: 5′GAGCAGGAGGGATTGTAGAAAAC3′ |
60 | 122 |
mmu_circRNA_27795 | F: 5′ GCCGGGAAACAATTAGAGG 3′ R: 5′ CAGAAGGCTCGTTCAGGATG3′ |
60 | 214 |
mmu_circRNA_35632 | F: 5′ GGAAGTTTGGCAGCAGAATC3′ R: 5′ CAATTCCGTAAGTCTGTGCATC3′ |
60 | 203 |
mmu_circRNA_009555 | F: 5′ AGTTCCACGTACTGTTCCTTT 3′ R: 5′ CTTGCTGTCTTTCTTGGCTGA3′ |
60 | 172 |
mmu_circRNA_29626 | F: 5′ GGACATCGTGGAGTGGTTGAA 3′ R: 5′ AGAGACCTTTGTGGAGGACAGC 3′ |
60 | 63 |
mmu_circRNA_37351 | F: 5′ TTGAGCCGAAGCCAGAGGA 3′ R: 5′ CGAGGTTGGTGGTCAGGAAA 3′ |
60 | 153 |