Figure 15.

Calcified drusen in GA donor eyes. Cross sections from the posterior pole of GA2 stained with GFAP (red), vimentin (green), and DAPI (blue) reveal subretinal deposits (asterisks in A–O) ensheathed by glial processes (arrows in A–C and F–H) positive for GFAP and vimentin (A–C, F–H, K–M). DIC of these same sections shows refractive spherules within these deposits (D, I, N). Adjacent sections stained with alizarin red S demonstrate that deposits contain calcium spherules (E, J, O). Calcium is also seen in BM. Toluidene blue–stained semithin sections from GA donor 2 taken near the border of RPE atrophy showing spherical calcium particles within a subretinal deposit (arrows) at low (P) and higher (Q) magnification. Ultrathin section (R) of calcium spherules (arrow) in the subretinal deposit shown in P and Q. The deposit (S) is surrounded by Müller cell (arrowhead) and astrocyte processes (asterisks).