Skip to main content
. 2023 Mar 3;9(9):eabo4616. doi: 10.1126/sciadv.abo4616

Fig. 1. Human CPC-sEV composition and miRNA content were not altered by miR-192-5p and miR-432-5p depletion.

Fig. 1.

(A) Venn diagram of top 50 most influential miRNAs identified from previously published studies (20, 21). miR-192-5p and miR-432-5p were identified from the intersection of the two independent studies using computational models to link CPC-sEV miRNA to cardiac repair in in vitro and in vivo models. (B) Biological pathway enrichment of miR-192-5p and miR-432-5p gene targets. (C) Schematic illustration of miRNA knockdown in sEVs produced from synthetic miRNA inhibitor–treated CPCs. (D) Nanoparticle tracking analysis and TEM imaging of sEVs with and without inhibitor treatment. Inserted images placed in the top right of each graph showed the corresponding sEVs. (E) Western blot of tetraspanin 9 and calnexin from CPC and sEV lysate, with and without inhibitor treatment. (F) Relative expression of miR-192-5p and miR-432-5p from CPCs and their sEVs with and without inhibitor treatment.